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KMID : 0545120080180101695
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Journal of Microbiology and Biotechnology
2008 Volume.18 No. 10 p.1695 ~ p.1700
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Development of Surface Plasmon Resonance Immunosensor Through Metal Ion Affinity and Mixed Self-Assembled Monolayer
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Lee Si-Ra
Sim Sang-Jun
Park Chul-Hwan
Gu Man-Bock
Hwang Un-Yeon
Yi Jong-Heop
Oh Byung-Keun
Lee Jin-Won
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Abstract
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An immunosensor based on surface plasmon resonance (SPR) with enhanced performance was developed through a mixed self-assembled monolayer. A mixture of 16- mercaptohexadecanic acid (16-MHA) and 1-undecanethiol with various molar ratios was self-assembled on gold (Au) surface and the carboxylic acid groups of 16-MHA were then coordinated to Zn ions by exposing the substrate to an ethanolic solution of Zn(NO3)2·6H2O. The antibody was immobilized on the SPR surface by exposing the functionalized substrate to the desired solution of antibody in phosphatebuffered saline (PBS) molecules. The film formation in series was confirmed by SPR and atomic force microscopy (AFM). The functionalized surface was applied to develop an SPR immunosensor for detecting human serum albumin (HSA) and the estimated detection limit (DL) was 4.27 nM. The limit value concentration can be well measured between ill and healthy conditions.
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KEYWORD
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Mixed self-assembled monolayer, surface plasmon resonance, immunosensor, metal ion, human serum albumin
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